tropicalis secretes high levels of Saps in a medium containing

tropicalis secretes high levels of Saps in a medium containing

bovine serum albumin as the sole source of nitrogen.[41] Sap expression in C. tropicalis during colonization of the oral epithelium is not associated with invasion and tissue damage.[51] Sap production has been studied preferentially in C. albicans and few reports have been found on Sap production Mitomycin C mw of non-albicans Candida spp. It is believed that there is a correlation between the expansion of SAP genes and the transition from commensal to pathogenic microorganisms. Non-pathogenic Candida spp. usually have fewer genes encoding Sap than opportunistic pathogenic species and this fact can be confirmed by gene sequencing these strains. However, this rule cannot be applied to species such as C. glabrata or C. krusei, which do not possess any SAP genes.[44] SAP genes are differentially involved in the development and maintenance of infections.[21] Expression of SAP1–SAP3 appears to be essential in mucosal infections and SAP4–SAP6 expression is essential in systemic infections.[21, 52, 53] The proteinases encoded by SAP9 and SAP10 appear to play a role in cell integrity, adhesion and cell separation after budding. In an infected host, Candida spp. are found in both hyphae and yeast forms. It is believed that hyphae formation is essential for fungal invasion, HM781-36B concentration as it assists in

the escape from the macrophage after phagocytosis.[41, 54] Some studies crotamiton in vitro have reported that SAP1–SAP3 are expressed in the yeast phase, whereas SAP4–SAP6 are expressed only in the hyphal phase (Fig. 2).[41, 55-58] It is believed that SAP2 has a functional role in invasion and spread of systemic infections.[52, 58, 59] The expression of these genes and the development of hyphae are not strictly linked, but are governed

by the same factors.[41] A further study on substrate specificity of Sap isoenzymes conducted by Aoki et al. [61] showed similar specificity among them. They were clustered into three groups according to substrate specificity. Sap7 and Sap10 showed high substrate specificity, whereas other Sap isoenzymes had broad substrate specificity. Interestingly, Sap4 to Sap6, which are coproduced in the hyphal form, may target similar host proteins. According to Ortega et al. [44], the pattern of SAP gene expression can be modified depending on the exposure conditions of the isolates. Physiological stress seems to promote increased secretion of Sap. Gene expression is variable and may be influenced by environmental conditions in vivo and by experimental conditions in vitro. Results of a study by White and Agabian [20] suggest that the cellular type controls the expression pattern of Sap isoenzymes. Studies on SAP gene expression identified seven genes as being differentially regulated in vitro (Fig.

Bone marrow-derived cells have the unique ability to differentiat

Bone marrow-derived cells have the unique ability to differentiate into target cells and promote healing activities. However, these abilities are expressed only in suitable environments. Human urethral sphincters with post-surgical ISD-related urinary incontinence have not been investigated to determine if the damaged regions provide such an environment that supports regeneration by bone marrow-derived cells. To achieve clinically

significant regeneration with these cells, it may be necessary to combine them with tissue engineering techniques that utilize scaffolds and/or growth factors.2 In any case, we clearly show that in rabbits the implantation of bone marrow-derived cells accelerates MK-8669 cost Selleck SAHA HDAC the recovery of freeze-injured urinary sphincters compared to cell-free injections. At 7 and 14 days after implantation, we determined if the cells organized into the reconstructed muscle layer structures are derived from the implanted autologous bone marrow-derived cells. The tissues are double-stained with GFP antibody in combination with striated muscle cell-, smooth muscle cell-, or myoblast-differentiation marker antibodies. At 7 days, some of the implanted cells identified by the presence of antibody-labeled GFP are simultaneously

positive for myoglobin Protirelin antibody. These double positive cells show that the implanted autologous cells differentiate into striated muscle cells. These differentiated cells are widely distributed within the reconstructed muscle layers (Fig. 4a). At 14 days after implantation, the double-labeled cells appeared to form contacts among themselves, creating striated muscle layer structures (Fig. 4b). Other GFP-positive implanted cells are also simultaneously positive for

SMA antibody. These cells show that the implanted cells differentiate into smooth muscle cells. Such cells are also widely distributed within the reconstructed muscle layers (Fig. 4c). Similarly, these double-labeled cells appear to form contacts among themselves, creating smooth muscle layer structures (Fig. 4d). In addition, the striated- and smooth-muscle differentiated cells contact non-GFP expressing muscle tissues that are presumably derived from the uninjured surrounding tissues. These cells are then integrated into the recovered muscle layers. We focus only on the implanted cells that maintained expression of GFP after implantation. At 7 days, the majority of both GFP and myoglobin, or SMA, or Pax7 double-positive cells are mononuclear. While we cannot definitively exclude the possibility of cellular fusion, the findings suggest that the number of these double-positive cells formed by cellular fusion is small.

Similar events may be initiated in T cells by ICs and complement

Similar events may be initiated in T cells by ICs and complement activation in autoimmune disorders. Syk has been a target for therapeutic intervention for autoimmune diseases. Syk-mediated signalling contributes to the altered T cell signalling [31]. In this report, we demonstrate that the FcγRIIIA/B receptor engagement by ICs on CD4+

T cells leads to the recruitment of the signalling subunit, the FcRγ chain, thus resulting in Syk activation. The learn more presence of soluble TCC enhances this signalling event. TCC in fluid phase by associating with vitronectin (S protein) becomes cytolytically inactive and is regarded as irrelevant. However, recent reports have shown that TCC induces functional activities such as kinin-dependent vascular leakage, activation of endothelial cells and induction of osteoprotegerin [16,32,33]. Vitronectin facilitates the cellular adhesion of soluble TCC, providing a mechanism to trigger cellular responses [34]. Previously, we have shown elevated levels of vitronectin associated with membrane attack complex (MAC) in lupus nephritis patients [23]. Our results point to a synergistic

role for TCC in IC-mediated Syk activation in CD4+ T cells. Such synergistic action of ICs and MAC in chemokine secretion during lung tissue injury has also been reported previously [35]. Binding of AHG (Fig. 1) and ICs purified from SLE Bcl-w to the peripheral CD4+ T cells establishes the interaction and a possible role of ICs in T cell responses. Previously, activation-dependent selleck screening library expression of FcγRII and FcγRIII receptors in the human T lymphocyte subpopulation has been observed [36]. This study showed a four- to 10-fold increase in the FcγRIII+ CD8+ T cell population in response to phytohaemagglutinin (PHA) treatment on day 3 post-stimulation [36]. Our results also point to a similar phenomenon, where FcγRIII+CD4+

T cells expanded in vitro using anti-CD3 and CD28, a total of more than 40% cells stained for FcγRIIIA/B in comparison to 10% directly from the PBMC. To explore whether ICs can influence the T cell physiology, we investigated the role of these complexes in Syk activation. Syk is a homologue of non-receptor tyrosine kinase ZAP-70. Syk is activated by FcRγ chain upon ITAM phosphorylation. Syk is expressed widely in both immune and non-immune cells [37,38]. Both DAP-12 and FcγR associate with Syk and mediate β-2 integrin signalling in neutrophils and macrophages [39]. Syk phosphorylation also occurs upon engagement of pathogen recognition receptors such as FcγR, CR3 and Dectin-1 [1]. Accumulating evidence points to Syk expression in subsets of T lymphocytes such as thymocytes, naive αβ T cells and intraepithelial γδ T cells, but not in proliferating and mature T cells [31,40].

These findings suggest that NKT cells can differentially regulate

These findings suggest that NKT cells can differentially regulate immune responses through the use of appropriate strategies depending on the local inflammatory environment 38. The differentiated IFN-γ-producing cells observed in experimental autoimmune encephalitis and uveitis may also play an important pathogenic role, AZD1152-HQPA chemical structure as the transfer of effector Th1 cells has revealed distinct disease patterns 17, 39. The presence of cells producing both IL-17 and IFN-γ in encephalitis 3 and experimental uveitis (our unpublished data)

also suggests that Th17 and Th1 cells are not mutually antagonistic and are representative of different aspects of pathogenesis in autoimmune disease. Human autoimmune diseases, including encephalitis and uveitis, have diverse spectrums of clinical diseases that are composed of various aspects of the immune response 40, 41. Therefore, CD1d-dependent invariant NKT cell-mediated regulation of different Th effector cells could provide a more ideal strategy for the control of human autoimmune disease caused by diverse pathogenic profiles. OT-II TCR transgenic mice, which express a TCR specific for OVA peptide (amino acid residues 323–339) in the context of I-Ab, were purchased from Jackson Laboratory (Bar Harbor, ME, USA).

CD1d−/− mice on a C57/B6 (B6) background have been described previously 20. Jα18−/− mice on a BL6 background were obtained from Dr. Masaru Taniguchi (RIKEN Research Center). IL-4−/−, IL-10−/−, and IFN-γ−/− mice on B6 background and B6 and B6.Thy1.1 NU7441 order mice were purchased from Jackson Laboratory. All mice were bred

and maintained in specific pathogen-free conditions at the animal facility of Seoul National University College of Medicine. All animal experiments were performed with the approval of the Institutional Animal Care and Use Committee (IACUC) at Seoul National University. Human IRBP peptide1–20 (GPTHLFQPSLVLDMAKVLLD) was synthesized by Peptron (Korea). Purified pertussis toxin and incomplete Freund’s adjuvant were purchased from Sigma (St. Louis, MO, USA). Mycobacterium tuberculosis L-gulonolactone oxidase strain H37RA was purchased from Difco (Detroit, MI, USA). α-Galcer was synthesized as described previously 20 and resuspended in 0.5% Tween-20 in PBS at a concentration of 220 μg/mL. OT-II mice were depleted of NK1.1+ cells by i.p. injection of an anti-NK1.1 antibody (PK136) 5 days and 2 days before being euthanized for the experiment (100 μg each day). Lymph node cells from OT-II mice (5×105) were stimulated with 0.2 μM OVA peptide in the presence of FACS-purified NKT cells (2×104). Th17 differentiation was initiated by the addition of 10 ng/mL of recombinant mouse IL-6 and 5 ng/mL of human TGF-β to the culture. NK1.1+ TCR+ cells were purified from hepatic MNC using a FACSAria (Becton Dickinson, USA).

It has been

It has been suggested that multifunctional CD4+T cells, able to produce simultaneously IFN-γ, IL-2 and TNF-α, are associated with protective immunity or a beneficial outcome in chronic infectious diseases, such as HIV [25–28] and HCV [29]. We therefore evaluated the quality of Th1 responses

induced by LbAg and LaAg in healed CL patients, based on their ability to secrete these three major Th1-related cytokines at the single-cell level. Using multiparametric flow cytometry, seven distinct populations of cytokine-producing cells can be delineated based on any of the possible combinations of IFN-γ+, IL-2+ and TNF-α+ producers, and the relative frequency of these distinct populations defines the quality of the Th1 response. The percentages of cytokine-producing cells were shown to be higher in the healed CL patient group than in healthy controls, and we were able to observe statistically significant differences between those groups for triple-positive (3+) multifunctional LY294002 nmr T cells (with both LbAg and LaAg), IFN-γ single-positive cells after LaAg stimulation and for IFN-γ+IL-2+ cells stimulated with LbAg (Fig. 2a). When comparing the quality of the Th1 response elicited by each Leishmania antigen evaluated we could observe that LbAg induces significantly higher percentages of multifunctional CD4+T cells and

IFN-γ+IL-2+ cells than LaAg stimulation in the healed CL patient group (Fig. 2a). The quality of the Th1 response was also evaluated by analysing the contribution of each phenotype in the total Th1 response, and is represented pictorially by pie charts (Fig. 2b). This kind of representation demonstrates clearly that LbAg induced a major proportion of multifunctional CD4+T cells (in red – 28% of the total Th1 response evaluated) and double-positive CD4+T cells Glutathione peroxidase (in blue – comprising 44% of the total Th1 response), while LaAg induced

predominantly single-positive cells (68%). More than half of the single-positive cells induced by LaAg were IFN-γ single-positive. In the control group, the majority of responsive cells were single-positives (>60%), and no major differences were observed concerning LbAg and LaAg stimulation. Having shown that LbAg induced higher cytokine production by CD4+T cells than LaAg in healed CL patients (Fig. 1b), we also investigated the relative cytokine concentrations produced by all distinct Th1 phenotypes induced by LbAg and LaAg, measured as the geometric MFIs. The highest MFI values for all three cytokines were found among triple-positive multifunctional CD4+T cells (both after LbAg and LaAg stimulation) (Fig. 2c) and a progressive decrease in the MFIs for all cytokines was observed as the degree of functionality decreased (3+ to single-positives). MFIs for IFN-γ and IL-2 from multifunctional T cells stimulated with LbAg were significantly higher than those obtained after LaAg stimulus (Fig. 2c).

One explanation could be that the cortical processes that are act

One explanation could be that the cortical processes that are actively working to update the familiar stimulus in their memory represent enhanced memory processes that could be seen in the VPC

as well, that is, a more robust or greater PSW as the reflection of memory updating could relate to a greater novelty preference. However, as a group, memory for the familiar stimulus after a 24-h delay is not yet solidified to the point that it is visible on behavioral testing alone. Although the HII sample was too small for testing similar relations, a preliminary analysis revealed that group and PSW interact to influence Day 2 novelty preference,

suggesting that different mechanisms might be underlying the relations between behavioral and electrophysiological measures of memory in the two groups. While prior 5-Fluoracil concentration studies have found both adolescents and adults with a history of early HII to be impaired on measures of visual recognition memory, delayed selleck chemicals llc recall, and tests of attention and executive function (Maneru, Junque, Botet, Tallada, & Guardia, 2001; Vargha-Khadem et al., 1997), the present preliminary findings for this group of infants experiencing mild-to-moderate HII suggest that while behaviorally (both on the VPC and on standardized cognitive assessment), these infants do not differ from typically developing infants at 12 months, the underlying neural mechanisms for memory and attention might be atypical. However, despite this pattern of similarities and differences between groups in the present study, an important set of limitations must be considered. First

and foremost, the HII results need to be interpreted with caution due to the small sample size. To increase the power of the present statistics for the VPC and ERP, a larger sample size is needed that can help elucidate how these tasks might differ as a function of perinatal HII. Further, perinatal HII is not a homogenous experience, Leukotriene-A4 hydrolase as can be surmised from Table 1, and therefore, a further limitation of the present work is that it was unable to more precisely group these infants into potentially meaningful subgroups, such as separating infants who did or did not undergo therapeutic hypothermia shortly after birth. With these limitations in mind, future work with this important population of infants is needed to expand the present findings and further explore the neural mechanisms underlying memory that might develop differently as a result of perinatal HII.

Trichostrongylus retortaeformis: The establishment, development a

Trichostrongylus retortaeformis: The establishment, development and survival of nematodes in the small intestine caused significant villous atrophy, increased crypt hyperplasia, reduction in the height-depth villus-crypt ratio and local recruitment of plasma cells, eosinophils and lymphocytes, compared to the controls

(For all Fisher’s exact test: P < 0·05). No significant changes in the intensity of the damage were observed with the course of the infection. Graphidium strigosum: Consistent focal glandular destruction, epithelial dedifferentiation and higher recruitment of eosinophils, lymphocytes and plasma cells were observed in the stomach tissue of infected compared to control individuals

(For all Fisher’s exact test: P < 0·01). Overall, selleck chemical both nematodes appeared to cause pathological damage by altering mucosa structure and recruitment of leucocytes to the site of infection. Trichostrongylus retortaeformis: The linear combination of IFN-γ, IL-4, IgA, IgG, eosinophils and lymphocytes, measured at the local site of infection (i.e. mucosa tissue or mucus of the SI-1 section), explained a large proportion of variation in the immune response to T. retortaeformis. The multivariate combination of these variables accounted for 64% of total variation in the first two principal components of a PCA (proportion of variance ± SD: PC-1 = 0·35 ± 1·44 and PC-2 = 0·29 ± 1·325). The first principal component was mainly driven by the effect of eosinophils (coeff. = 0·525), lymphocytes (0·562) and the opposite effect of IFN-γ CYTH4 (−0·500). Obeticholic Acid The second principal component was affected by mucus IgA (−0·570) and IgG (−0·567) and the opposite contribution of IL-4 (0·456). Ct values are inversely related to cytokine expression, so that, high values -or a positive correlation- represent low cytokine expression and vice-versa. Changes in T. retortaeformis abundance were examined in relation to the estimated principal components, and a significant positive relationship

was found with the second principal component (coeff. ± SE = 0·601 ± 0·274, d.f = 38, P < 0·05, Figure 7a), indicating that a decrease in parasite abundance was associated with an increase in IL-4 and antibody responses. No significant association was observed with the first principal component. Nematode abundance was tested against the variables selected in the PCA, and the results confirmed that T. retortaeformis infection was negatively associated with IL-4 (coeff. ± SE: 0·718 ± 0·348, P < 0·05) and positively associated with IFN-γ (−0·569 ± 0·247, P < 0·05). A negative relationship was also found with mucus IgG and mucosa eosinophils and lymphocytes (second-order interaction with time, for all P < 0·05, IgG: P = 0·056), while a positive association was observed with mucus IgA alone (P < 0·01).

First, we evaluated the qualities of the BMT model Multilineage-

First, we evaluated the qualities of the BMT model. Multilineage-full

chimerism in the case of BMT using fully allogeneic BMC and multilineage-mixed chimerism in the case of BMT using mixed BMC were confirmed in the PBL of all recipients prior to tumour inoculation (Fig. 4A and data not shown). As previously reported, a small population of recipient-derived radio-resistant T cells were detected in recipients transplanted with BL6 BMC (the lower right dot plot in Fig. 4A). However, these T cells showed no alloresponses [29] and so should not affect the survival time of injected allogeneic DC. The B-cell and myeloid lineage chimerism selleck kinase inhibitor in the PBL was complete in the recipients transplanted with BL6 BMC (the upper right dot plot in Fig. 4A and data not shown). This suggests that the bone marrow haematopoietic cells had been completely replaced by donor-derived BMC. We also assessed the chimerism of the DC in the lymph nodes (inguinal and axillary) and tumours of the B/c recipients. A significant Dabrafenib price percentage of recipient-derived DC was detected in the lymph nodes of recipients transplanted with BL6 BMC (Fig. 4B). More than 60% of residual B/c recipient-derived H-2Kd positive DC in the cutaneous draining lymph nodes expressed DEC205 and I-Ad high, both markers

found on Langerhans cells (data not shown) [34]. This suggests that these cells were derived from Langerhans cells repopulated from the radio-resistant progenitor cells in the cutaneous niche [35]. Because Langerhans progenitor cells in the cutaneous niche are destroyed by any contaminating donor-derived T cells

that mediate graft-versus-host disease (GVHD) [35], the presence of host-derived Langerhans cells in the draining lymph nodes in this study suggests that the T-cell depletion of the donor BMC was complete. This is supported by the fact that we observed no GVHD response in these mice; this observation was confirmed by histological analysis at autopsy (data not shown). To the contrary, almost all tumour-associated DC were positive for H-2Kb and negative for H-2Kd (Fig. 4B). This suggests that the tumour-associated DC were derived from donor BMC. Taken together, we judged that the developed BMT model was qualitatively appropriate to evaluate the three factors individually in ITADT. Surprisingly, we found that ITADT else using BL6 DC showed a significant antitumour effect in terms of tumour growth suppression as well as ITADT using B/c DC in recipients of mixed BMC (BL6+ B/c B/c), despite the MHC incompatibility of the injected BL6 DC (Fig. 4, middle graph). However, ITADT using BL6 DC did not show any significant antitumour effect in recipients of BL6 BMC, despite the fact that these recipients were tolerant to BL6 (Fig. 4, left-hand graph), while ITADT using B/c DC did show a significant antitumour effect in recipients of BL6 BMC. In recipients of syngeneic BMC (B/c B/c), ITADT using B/c DC showed a significant antitumour effect.

In conclusion, in this study, an altered peptide ligand p321-1Y9L

In conclusion, in this study, an altered peptide ligand p321-1Y9L (YLIGETIKL) was identified with enhanced binding stability and immunogenicity derived from the native peptide in COX-2. Our results showed that p321-1Y9L could induce more potent CTL response in vitro and in vivo, which could lyse tumour cells in COX-2-specific and HLA-A2-restricted manners. This CTL epitope could serve as an attractive component of peptide-based vaccines to the immunotherapy of cancer patients. This work was supported by grants from the National Natural Science Foundation of China (No. 81172893, 30901362, 81000673), and the National Science and Technology Major Projects

of New Drugs Y-27632 molecular weight (2012ZX09103301-023). There are no conflicts of interest. “
“Blood levels of regulators of the complement system in preterm babies were reported in few studies only. The aim of this study was to set up a complement profile in premature and term babies focusing on the development of blood

levels of MBL, key regulatory proteins check details and on classical pathway activity, which may allow an estimation of potential susceptibility to infection. Complement activity (CH50), levels of mannan-binding lectin (MBL), complement regulators (factors H and I, C1 inhibitor, properdin) and C3a as marker of complement activation were assessed in three groups of healthy newborns: (1) prematures (≤34 weeks); (2) late prematures (>34–<37 weeks) and (3) term neonates (≥37 weeks). CH50 increased

with gestational age with lower Baricitinib titres in cord blood than in day 5 post-delivery venous blood. MBL concentrations were not significantly different among groups. Quantitative and functional C1 inhibitor were below adult normal range in prematures <34 weeks and lower in cord blood as compared to day 5. Factor I, factor H and properdin remained below adult values in all groups. Low C3a levels excluded that low complement titres were due to activation-induced consumption. These results demonstrate the relative immaturity of the complement system and its regulation, especially in premature infants. "
“We assessed the mucosal response of previously infected hamsters to low-dose challenge with the hookworm, Ancylostoma ceylanicum. Hamsters were assigned to five treatment groups (Groups 1–5, respectively): naïve, controls; uninterrupted primary infection from day 0; infected, but treated with anthelmintic on day 35 p.i.; challenge control group given only the second infection on day 63; infected initially, cleared of worms and then challenged. Animals were culled on days 73 and 94 (10 and 31 days after challenge), but additional animals were culled from Group 5 on days 80 and 87. The results showed that villus height declined markedly and progressively over time after challenge in Group 5, whilst depth of the Crypts of Lieberkühn and number of mitotic figures in the crypts increased.

The samples were incubated at 37 °C in a humidified 5% CO2 incuba

The samples were incubated at 37 °C in a humidified 5% CO2 incubator for 24 h. On the second day, the tubes were centrifuged at 3000 rcf for 10 min CHIR-99021 price and the

plasma was collected and stored at 4 °C until IFN-γ assay was performed using ELISA. The optical density of each test was read using a 450-nm filter with a 620-nm reference filter with an ELISA plate reader. The results were interpreted as positive, negative or indeterminate using QFT-GIT analysis software (QFT-GIT; Cellestis Ltd). If the IFN-γ secretion in response to TB antigen, after subtracting nil control IFN-γ, was ≥ 0.35 IU mL−1, it was considered positive for QFT-GIT; and if the value was < 0.35 IU mL−1, it was considered negative. If the negativity was associated with poor phytohaemagglutinin (PHA) response (i.e. IFN-γ secretion in response to mitogen was < 0.5 IU mL−1), it was considered as indeterminate or invalid result for QFT-GIT. The subjects with IFN-γ secretion > 8.0 IU mL−1 in the nil control samples were also considered indeterminate for QFT-GIT.

Immediately following blood collection from the right hand of each participant, 0.1 mL (2 T.U/0.1 mL) Tuberculin PPD RT23 (Statens Serum Institute, Copenhagen, Denmark) was administered intradermally in the middle third of the left forearm by an experienced nurse. The diameter induration transverse to the long Bortezomib cost axis of the forearm was measured between 48 and 72 h using a flexible plastic ruler. A diameter of skin induration ≥ 10 mm was considered positive for tuberculin skin test (TST). In all, 40 mL pleural fluid was concentrated by centrifugation at 10 000 g at 4 °C for 20 min. Then the pellet was re-suspended in 1 mL sterile distilled water and stored at −20 °C for DNA extraction. Three sputum specimens (spot-morning-spot) from each participant were collected and M.tb was detected with an AFB smear using

the Ziehl–Neelsen method and mycobacterial culture in both Lowenstein Jensen (Biomerieux Inc., L’Etoile, France) and MGIT tubes (BD BACTC MGIT 960 system). The DNA from pleural fluid pellet suspension was extracted using the DNeasy acetylcholine Blood & Tissue Kit (Qiagen, Hilden, Germany). Nested PCR was performed using the Seeplex® MTB Nested ACE Detection kit according to the manufacturer’s instructions. This detection kit utilizes multi-target (IS6110 and MPB64) instead of single-target PCR for specific detection of M.tb. A mixture of bacterial clones and internal clones were used as positive controls. To eliminate any possibility of cross-contamination from the positive controls, the amplification sizes of the positive control PCR products (810 and 745 bp) were designed differently from those of the specimen PCR products (255 and 190 bp). The statistical analysis was performed with graphpad prism software (version 5.01; GraphPad Software, Inc.) and medcalc Software (Version 11.4.2; MedCalc Software bvba).