Nevertheless, deeper into the gingival connective tissue, gingipain concentrations become gradually lower and stimulate, rather than inhibit, inflammation. This may in

turn induce connective tissue and bone destruction, which are the hallmarks of periodontitis. It is evident that P. gingivalis has developed mechanisms to invade and persist into the host, by astutely Panobinostat purchase adapting to its local niche. Its paradoxically opposing (stimulatory vs. inhibiting) effects on innate immune and inflammatory responses aim to subvert host defence mechanisms, in order to facilitate its survival in the tissues (Hajishengallis, 2009; Hajishengallis & Lambris, 2011). The net effect of this deregulated equilibrium is likely to determine if site-specific disease progresses beyond or remains at stationary phase. Whether inflammation is beneficial for P. gingivalis may depend on the stage of its establishment in the host (Hajishengallis, 2009; Pathirana et al., 2010). At early stages, suppression of inflammation and evasion of host recognition would aid P. gingivalis in colonizing, invading and establishing at the targeted site. At later stages, once P. gingivalis is well established, inducing inflammation may facilitate its increased demands in nutrients. Alternatively, P. gingivalis may

induce a ‘nonproductive inflammation’, one that fails to eliminate it, yet is sufficient to induce mediators of tissue destruction (Hajishengallis, 2009). Finally, as periodontitis is of polymicrobial

nature, it is reasonable to consider the role of different bacterial species within the context of (subgingival) Janus kinase (JAK) biofilm GPCR & G Protein inhibitor communities. Hence, P. gingivalis is likely to function in concerted action with other species, to their mutual benefit. For instance, complement manipulation by P. gingivalis may denote a coevolution strategy to support other species present in the biofilm, which may reciprocally provide further colonization opportunities and nutrient availability to P. gingivalis. Subsequent changes in the local microenvironment can differentially regulate expression of its virulence factors, and hence the proinflammatory or anti-inflammatory potentials of P. gingivalis. This is strongly indicated by recent evidence demonstrating that even at low abundance, this species qualitatively and quantitatively affects the composition of the oral commensal microbiota, which are in turn required for P. gingivalis-induced inflammatory bone loss (Hajishengallis et al., 2011). For these reasons, P. gingivalis is now considered a ‘keystone’ species in subgingival biofilms (Honda, 2011). This work was supported by the Institute of Oral Biology, Center of Dental Medicine, University of Zürich. “
“d-Xylulokinase catalyzes the phosphorylation of d-xylulose in the final step of the pentose catabolic pathway to form d-xylulose-5-phosphate.