2 °C, which confirms that strain MY14T does not belong to the genospecies O. flavum. The DNA–DNA relatedness studies between strains ND5 and H. saxobsidens NS11T, the strain with the highest 16S rRNA (99.8%) and cpn60 (98%) gene sequence similarity with ND5, showed that ΔTm values were 6.6 °C. However, the ΔTm value between H. glaciei UMB49T and strain ND5, sharing 99.6% 16S rRNA and 97.6%cpn60 gene sequence similarity, was 1 °C, which is well below the 5 °C cut-off point recommended for the delineation of species (Wayne Poziotinib price et al., 1987; Rosselló-Mora & Amann, 2001). Similar observations of contrasting high 16S rRNA gene sequence similarity (99.6–99.8%) and low (3–57%) DNA–DNA relatedness have

been reported among all described Herminiimonas-type strains (Kämpfer et al., 2006; Muller et al., 2006; Lang et al., 2007; Loveland-Curtze et al., 2009). On the basis of the results described above, it can be concluded that the strain ND5 (=NBRC 102664, =CCM 7665) is another strain of H. glaciei and strain MY14T represents a novel species within the genus Oxalicibacterium, for which the name O. solurbis sp. nov. is proposed. Oxalicibacterium solurbis (sol.ur’bis. L. n. solum, soil; L. n. urbs urbis, a city; N.L. gen. n. solurbis, of city soil, where the type strain was isolated). Gram-negative, small rods 0.4–0.5 × 0.8–1.2 μm (mean cell volume 0.07 μm3), motile by

polar flagella. At low temperatures around 4 °C, elongated cells are sometimes observed. No spores were observed. Oxidase FDA approved Drug Library datasheet and catalase reactions are positive. Colonies on nutrient agar (Oxoid CM3) with lactate are slightly yellow pigmented. Forms smooth, glistening,

raised, opaque with entire edges; the diameter is up to 0.5–1 mm after 3 days of incubation at 28–30 °C. Growth occurs at 4 °C and up to 37 °C, but not 42 °C. Optimum growth occurs at 37 °C and pH 8.0. Grows in media containing 5% NaCl. The specific growth rate (μ) under optimum conditions with lactate was 0.14 h−1. No acid produced from glucose. Nitrate is not reduced to nitrite. Negative for indole production, arginine dihydrolase, urease, esculin, casein Anacetrapib and gelatine hydrolysis and β-galactosidase. Sugars and alcohols were not utilized; utilizes fumarate, glycolate, dl-lactate, l-malate, malonate (weak), pyruvate, succinate, oxalate, l-alanine and l-glycine. Other differential characteristics are given in Table 1. The main fatty acids are C16:0, C17:0 cyclo, C19:0 cyclo ω8c and C10:0 3-OH. The major quinone system is ubiquinone Q-8. The major phospholipids are phosphatidylethanolamine and phosphatidylglycerol. The G+C content of DNA is 63.3 mol%. The type strain, MY14T (=NBRC 102665T, =CCM 7664T), was isolated from a soil sample using the membrane-filter enrichment technique. We are grateful to Dr Jean P. Euzéby, for his help with the Latin nomenclature of the species epithets. Thanks are also due to Dr J. Loveland-Curtze for supplying the type strain of H.