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Authors’ contributions YC, JGM and JAJ conceived the study. YC and JD designed and performed the experimental works. YC and JAJ drafted the manuscript. All authors read and proved the final manuscript.”
“Background Salmonella enterica serovar Typhimurium (S. Typhimurium)

is an important pathogen causing gastroenteritis in humans [1]. Salmonella is able to form biofilms on both biotic and abiotic surfaces. Growth in such biofilm structures increases the resistance against antibacterial treatments and enhances Screening Library price their spread and persistence outside the host [2]. Also, contamination of processed foods in industrial plants is often due to biofilm formation on both food and food-contact surfaces [3]. In some bacterial species, it has been reported that biofilm formation is partially regulated by a communication system called quorum sensing, more specifically depending on the quorum sensing synthase enzyme LuxS and the signaling molecule autoinducer-2 (AI-2) produced by LuxS [4–9]. In the case of Salmonella Typhimurium, it has been reported that biofilm formation is affected by mutating the luxS gene [10–12]. However,

De Keersmaecker et al. [10] showed that, although genetic complementation could be accomplished, the biofilm forming phenotype could not Afatinib be rescued by the addition of synthetic DPD, which non-catalytically is converted to AI-2. This suggested that AI-2 is not the actual signal involved in the formation of a Salmonella Typhimurium biofilm. Similarly, Karavolos et al. [13] reported altered flagellar phase variation in a S. Typhimurium luxS deletion mutant independent of quorum sensing signals. In order to further reveal the exact role of the luxS region in S. Typhimurium biofilm formation, we analyzed additional S. Typhimurium luxS mutants for their biofilm phenotype. We show that the S. Typhimurium biofilm formation phenotype is dependent on the sRNA molecule MicA, encoded in the luxS adjacent genomic region, rather than on LuxS itself. Results Phenotypic analysis of different luxS mutants Previously, we reported that a S. Typhimurium SL1344 luxS mutant lacking the entire LuxS coding sequence – from start to stopcodon – (CMPG5602) is unable to form a mature biofilm [10].