amarus (46.92 mg GAE/g) had maximum phenolic GSK2118436 ic50 content and Cissus quandrangularis (8.18 mg GAE/g) had least phenolic content. P. amarus was followed by C. aromaticus (42.82 mg GAE/g), L. aspera (29.41 mg GAE/g) and A. paniculata (17.11 mg GAE/g). The results revealed that P. amarus showed significant flavonoid and phenolic content, which is correlated with the earlier reports. 11 In this study, the phenolic compounds were assessed by Folin–Ciocalteau

reagent that does not give the complete picture of phenolics, however this assay will help to categorize the extracts based on their antioxidant potential. 8 The phenolic content of the medicinal plants vary considerably which may be due to the high solar radiation and temperature. 12 The primary characterization of scavenging ability of the plant extracts has been studied using a stable free radical DPPH. The results of radical scavenging activity of all the medicinal plants are shown in Fig. 3. Among the plants analyzed, BI 6727 price the highest DPPH radical scavenging activity was found in the leaves of L. aspera (75.06%), whereas it was lower in C. quandrangularis (42.86%). Many published data showed that phenolic compounds are responsible for the antioxidant

activity of the plants. 13 and 14 In contrast, despite the high flavonoid and phenolic content in Phyllanthus, its DPPH radical scavenging activity was really low, suggesting that the antioxidant activity of the plant extract may not be due to the specific

group of secondary metabolites like polyphenolics, which may be due to the combined groups of metabolites. 15 and 16 The antioxidant power of the medicinal plant extracts were assessed by FRAP assay. The DNA ligase FRAP values of all the medicinal plant extracts were given in Fig. 4. Ferric Ion Fe (II) reducing ability had marked differences among the plants and it was maximum in P. amarus (12.68 mM/g) and lowest in L. aspera (2.11 mM/g). With regard to FRAP values, Phyllanthus showed remarkable reducing power as compared to the other medicinal plants tested. By using FRAP assay, several groups reported the reducing power of other medicinal plants like Ocimum, A. paniculata and Cissus quadrangularis. 17, 18 and 19 The correlation coefficients Libraries between the radical scavenging activity and total flavonoids/phenolics were calculated. The DPPH radical scavenging activity did not correlate with flavonoid (r = 0.518, p > 0.05) and phenolic content (r = 0.412, p > 0.05). Also there is no significant linear correlation was found between the FRAP values with flavonoid (r = 0.449, p > 0.05) and phenolic content of the medicinal plants tested (r = 0.429 p > 0.05). Although there are some reports 20 and 21 showing a high correlation between the radical scavenging activity and phytochemical content, other authors 15 have found a low correlation. In the present study, no linear correlation was observed between the phytochemical content and antioxidant activity.