We found that although the HPA axis response during restraint of both previously stressed groups were higher than stress-naive rats and not different from each other, lack of control over the tailshock experience led to an increase in restraint-induced struggling behavior of the IS rats compared to both stress-naive and ES rats. Additionally, c-fos expression in the basolateral amygdala was increased selectively in the IS group, and relative c-fos mRNA expression in the basolateral amygdala positively correlated with struggling behavior. Restraint-induced Adriamycin manufacturer c-fos expression in the medial prefrontal cortex, a brain area critical for mediating some of the differential neurochemical and
behavioral effects of ES and IS, was surprisingly similar in both ES and IS groups, lower than that of stress-nave rats, and did not correlate with struggling behavior. Our findings indicate that basolateral amygdala activity may be connected with the differential effects of ES see more and IS on subsequent behavioral responses to restraint, without contributing to the concurrent HPA axis hormone response. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.”
“Children with constitutional trisomy 21 or Down’s syndrome (DS) are predisposed to develop myeloid leukemia (ML)
at a young age. DS-ML is frequently preceded by transient leukemia (TL), a spontaneously resolving accumulation of blasts during the newborn period. Somatic mutations of GATA1 in the blasts of TL and DS-ML likely function as an initiating event. We hypothesized that the phenotypic difference between TL and DS-ML is due to a divergent functional repertoire of the leukemia-initiating cells. Using an NOD/SCID model, we found that cells initiating DS-ML engrafted, disseminated
to distant bone marrow sites, and propagated the leukemic clone in secondary recipients. In contrast, TL cells lacked the ability to expand and to migrate, but were able to persist in the recipient bone marrow. We found some Thiamet G evidence of genomic progression with 1 of 9 DS-ML samples and none of 11 TL samples harboring a mutation of N-RAS. The findings of this pilot study provide evidence for the functional impact of second events underlying the transformation of TL into DS-ML and a needed experimental tool for the functional testing of these promoting events. Leukemia (2010) 24, 1012-1017; doi:10.1038/leu.2010.30; published online 11 March 2010″
“Previous studies have demonstrated that merlin acts as a tumor suppressor by blocking Ras-mediated signaling. However, the mechanism by which merlin controls cell proliferation has remained obscure. Here we show that merlin deficient tumors exhibited loss of p21, concomitant with elevated CDKs/cyclin D1 levels in sporadic vestibular schwannomas (VS) from clinic patients.