Turk J Phys Med Rehab 2012;58:103-8.”
“PURPOSE: To evaluate the effect of complete destruction of lens epithelial cells (LECs) in the capsular bag on intraocular lens (IOL) stability. SETTING: School of Biological Sciences, University of East Anglia, Norwich, United Kingdom. DESIGN: Comparative evaluation. METHODS: An in vitro organ culture model using the bag zonule ciliary body complex Selleckchem Copanlisib isolated from fellow human donor eyes was prepared. A capsulorhexis and fiber extraction
were performed, and an Acrysof IOL was implanted. Preparations were secured by pinning the ciliary body to a silicone ring and maintaining it in 6 mL Eagle minimum essential medium supplemented with 5% v/v fetal calf serum and 10 ng/mL transforming growth factor-beta 2 for 3 weeks or more. One bag of each pair was treated with 1 1,1,M thapsigargin to destroy all LECs. Observations of LEC growth were captured by phase-contrast microscopy, IOL stability by video microscopy, and endpoint analysis through scanning electron microscopy and immunocytochemistry. RESULTS The LECs in control capsular bags migrated centrally, closing the bag and fixating the IOL between the anterior and posterior capsules, as seen clinically. These events were not observed in the thapsigargin-treated group. After a period
of controlled orbital movement, the IOL in the control group stabilized quicker than in the treated bags. There was no IOL rotation PARP inhibitor drugs in the bag; however, the IOLs in the treated group rocked with axial movement. CONCLUSIONS: The LECs appeared to aid stabilization of current IOL designs in the capsular bag. The results have clinical implications for IOL design and for strategies to prevent posterior capsule MK 1775 opacification. (C) 2014 ASCRS and ESCRS”
“To evaluate if clinically usable estimates of physical performance and level of habitual physical activity are associated with fall risk in elderly men. A population-based
sample of 3014 randomly selected men aged 69-80 years was recruited to medical centers in Gothenburg, Malmoe, or Uppsala. The level of physical activity and self-reported falls during the preceding 12 months was evaluated using a questionnaire. The physical performance ability was estimated by measurements of handgrip strength, a timed stands test, a 6-m walking test and a 20-cm narrow walk test. Falls were reported in 16.5% of the men. Fallers performed 6.2 +/- 19.0% (mean +/- standard deviations; S. D.) less in right handgrip measures, 8.8 +/- 40.6% slower in the timed stands test, 6.8 +/- 30.8% slower in the 6-m walking test, and 5.3 +/- 28.8% slower in the 20-cm narrow walk test (all p < 0.001, respectively). The odds ratio for falls among men who performed < -3 S. D. or failed compared to the mean (+1 S. D. to -1 S. D.) in the timed stands test was 3.41 (95% CI 2.31-5.02; p < 0.001) and 2.46 (95% CI 1.80-3.34; p < 0.001) in 20-cm narrow walk test.