The recording chamber and eye coil were attached during surgery with sterile procedure with approaches described before ( Ramachandran and Lisberger, 2005) with the monkey

under anesthesia with isofluorane. After surgery, monkeys received analgesics for several days and careful monitoring by veterinary staff. All experimental procedures and protocols used were approved by the Institutional Animal Care and Use Committee of University of California, San Francisco and are in accordance with use and care guidelines established by the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Horizontal and vertical eye positions were sampled at 1 kHz and passed through an analog differentiator with a cutoff www.selleckchem.com/screening/anti-diabetic-compound-library.html of 25 Hz to produce the corresponding eye velocity traces. Quartz shielded tungsten electrodes

(Thomas Inc.) were lowered anew each day into the frontal eye fields. FEFSEM neurons were identified by direction-tuned activity during smooth pursuit and weak or nonexistent responses to saccades or 3-Methyladenine changes in eye position. Spike waveforms were retained with a threshold crossing criterion and were sorted into single units based on waveform shape and the absence of refractory period violations defined as two waveforms occurring within 1 ms. For a typical recording session, the waveforms from recorded neurons were three to ten times the amplitude of the background noise. Sorted waveforms were converted into spike trains with a temporal precision of 1 ms. All behavioral experiments took place in a dimly lit room. Visual stimuli were displayed on a BARCO monitor (model number CCID 7651 MkII) that was placed 40 cm from the eye and subtended 61° × 42°

of the visual field. Targets were white squares measuring 0.5° along each side. Target motions were presented in discrete trials. Each trial started with a stationary fixation target at the center of the screen for an interval that was randomized between 500 and 1000 ms. Targets then underwent standard step-ramp motion in an unpredictable direction for 750 ms, and then stopped Cediranib (AZD2171) for 500 ms in a second fixation period. For step-ramp motion, the step size was chosen to minimize saccades during pursuit onset and typically ranged between 2° to 3°, depending on the initial direction of target motion. To successfully complete a trial and receive a water reward, monkeys were required to keep their eyes within a window centered on the target. The window was 1.5° × 1.5° during fixation, 3° × 3° during smooth target motion, and 5° × 5° for 300 ms after an instructive change in target direction. For tests of neural responses to passive visual stimuli, monkeys fixated a small square target centered in an invisible square aperture that was 5° long on each side. The aperture contained 10 dots that moved with 100% coherence at 5°/s in one of the four cardinal directions.