Subcortical tissue thin, a loose t intricata of thin-walled, hya

Subcortical tissue thin, a loose t. intricata of thin-walled, hyaline hyphae (3–)4–7(–9) μm (n = 15) wide. Subperithecial tissue a dense hyaline t. angularis–epidermoidea of isodiametric subglobose or angular, thin-walled cells (4–)12–44(–63) × (3.5–)6–15(–19) μm (n = 30), becoming smaller towards the stroma base and intermingled with hyphal elements. Asci (68–)72–86(–98) × (3.5–)4.0–4.8(–5.2) μm, stipe (5–)7–20(–28) μm long (n = 30); no croziers apparent. Ascospores hyaline, finely verruculose, cells dimorphic, but often similar, distal cell (2.4–)2.6–3.3(–4.3) × (2.4–)2.5–3.0(–3.6) μm, l/w (0.9–)1.0–1.2(–1.4) (n = 70), subglobose, sometimes slightly tapered towards upper end, proximal

cell (2.4–)3.0–3.7(–4.5) × (2.0–)2.2–2.6(–3.2) μm, l/w (1.0–)1.2–1.5(–1.9) (n = 70), wedge-shaped or oblong, broadly rounded at lower end. Anamorph on the natural Selleck PXD101 substrate (WU 24044): White hairy tufts on wood, partly in close association with stromata, in circular to oblong, confluent patches to 15 mm long, with long sterile elongations when immature. Main axes 3–5 μm wide, with short branches in right angles, loosely disposed or pachybasium-like, i.e. richly and densely branched, with dense whorls of 2–5(–6) phialides on 1–2 celled branches 3–4(–7) μm wide; branching points often thickened. NVP-HSP990 Phialides

(4.2–)4.7–8.2(–12.0) × (2.5–)2.7–3.2(–3.5) μm, l/w = 1.5–2.8(–4.5), (1.4–)2.0–2.7(–3.0) μm wide at the base (n = 30), plump, short and thick, ampulliform or lageniform, widest in or below the middle. Conidia (2.2–)2.5–3.2(–3.7) × 1.7–2.0(–2.5)

μm, l/w = 1.2–1.5(–1.7) (n = 30), hyaline, ellipsoidal or oval, smooth, with one or few guttules. Cultures and anamorph: optimal growth at 25°C on all media, no growth at 35°C. On CMD after 72 h 5–7 mm at 15°C, 7–10 mm at 25°C, 3–10 mm at 30°C; mycelium covering the plate after 3–6 weeks. Colony characteristic, forming silky, fan-shaped lobes, with little mycelium on the agar surface, finely but distinctly zonate; hyphae Vorinostat purchase narrow, soon degenerating in the centre. Aerial hyphae inconspicuous, but sometimes appearing in loose, irregular, sterile or fertile tufts mostly in distal or lateral regions of the colony, on plates entirely covered by mycelium. After ca 6 days often characteristic colourless to white crystals appearing on the surface, growing to 0.5–1.5 mm diam, sometimes appearing as oily drops inside the agar; in some isolates or after several transfers no crystals formed. Autolytic activity and coilings variable, usually inconspicuous. No distinct odour detected. Either no diffusing pigment formed or a diffuse greyish yellow, golden- or yellow-brown, 4B4–7 to 5CD7–8, unevenly distributed pigment noted. Chlamydospores 5–19(–29) × (5–)6–15(–17) μm (n = 10), rare, terminal and intercalary, globose, pyriform or irregular.

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