Pretreatment of PC12 cells with PI3k/Akt and MEK1/2 inhibitors, U0126 and LY294002 paid off the phosphorylation of Akt and ERK1/2 in luteolin treated cells. It had been shown previously that certain correlations exist in between chemical screening cell differentiationAChE activity and ERK1/2 phosphorylation. In this respect, we evaluated statistical correlations between biochemical indices andmorphological variables caused by luteolin in PC12 cells, applying linear regression and nonparametric Spearmans rank correlation coefficient. Interestingly, we found that Spearmans list coefficient and the coefficient of determination indicate although the relationship with fusiform phenotype cells was poor, a positive and strong connection between the proportion differentiated cells, the proportion of cells with neuritis and biochemical indices. There is increasing evidence supporting the use of nonpeptidic neurotrophins against neurodegenerative disorders. Recently, interest has been centered on phytochemicals, including polyphenolic compounds, which can be in a position to induce neuronal differentiation and control chemicals characteristics in CNS. Luteolin is a well known reactive oxygen species scavenger and serves as an anti inflammatory, antioxidant, and anticancer agent. In animal experiments, luteolin has been proven to be blood?brain permeable substance with encouraging advantage on CNS and the treatment of neurodegenerative diseases. We formerly showed that luteolin may be one of many active materials in the beginning of neurogenic stimulated activities of Rosmainus officinalis. In our research, Lymph node we demonstrated that luteolintreated PC12 cells quit growth and got lots of differentiated phenotypic properties of sympathetic nerves including neurite outgrowth and escalation in cholinergic activities through the service of ERK1/2 and Akt. The direct way of study induced differentiation and cholinergic activities in PC12 cells suggested that luteolin is really a powerful cholinergic enhancement. Dizocilpine MK 801 Actually, luteolin addressed PC12 cells showed a substantial increase in neurite outgrowth with correlated increase of AChE activity, full choline and acetyl-choline levels. Luteolin probably will exert an impact much like that of NGF. AChE activity has been described by many studies to correlate with neuronal differentiation. Acetyl-choline is one of the major modulators of brain functions and it’s the major neurotransmitter at the peripheral nervous system. It is the key marker needed for cholinergic neurotransmission in the central and peripheral nerve system mainly in the basal forebrain cholinergic neurons and striatal neurons. Recently, it has been noted by Dagyt? et al. that in case of depression, the impairment of arousal and conduct is correlated with loss in cholinergic functions in CNS. Also, Mufson et al.