Mol Cancer Ther 2007, 6:2127–2138.PubMedCrossRef 50. Davis CD, Uthus EO: DNA methylation, cancer susceptibility, and nutrient interactions. Exp Biol Med (Maywood) 2004, 229:988–995. 51. Huang J, Plass C, Gerhauser C: Cancer chemoprevention by targeting the epigenome. Curr Drug Targets 2011,12(13):1925–1956.PubMedCrossRef 52. Trabelsi N, Oueslati S, Falleh H, Waffo-Taguo P, Papastamoulis Y, Mârillon J-M, Abdelly C, Ksouri R: Isolation of powerful antioxidants from the medicinal LY2874455 halophyte Limoniastrum guyonianum. Food Chemistry 2012, 135:1419–1424.PubMedCrossRef 53. Fini L, Selgrad M,

Fogliano V, Graziani G, Romano M, Hotchkiss E, Daoud YA, De Vol EB, Boland CR, Ricciardiello L: Annurca apple polyphenols have potent demethylating activity and can reactivate silenced tumor suppressor genes in colorectal cancer cells. J Nutr 2007, 137:2622–2628.PubMed 54. Unoki M, Nishidate T, Nakamura Y: ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain. Oncogene 2004, 23:7601–7610.PubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions MK, MA, CB and MM designed the experiments and the draft. CDM, JPG, LCG, KG and YM equally contributed to the writing the article. All authors read and approved the final manuscript.”
“Background Geneticin mouse Lung cancer is one of the leading

causes of cancer-related mortality in the world, and the incidence rates are increasing in many countries [1]. Although the prognosis is improving, the 5-year overall survival rate of lung cancer patients is still only approximately 16% [2]. In order to improve survival outcome, it is important to detect and surgically remove lung cancer at an early stage. Currently, the cancer stem cell (CSC) theory proposes that tumors contain a small subpopulation of CSC, which is responsible for tumor growth, invasion and metastasis [3]. CSC and normal tissue stem cells PDK4 share

important characteristics: self-renewal, multipotency and unlimited proliferation, and potentially overlapping molecular mechanisms [4, 5]. In human adult tissues and tumors, several hundred stem-cell-associated markers have been identified. In lung cancer, the common stem-cell-associated markers include Bmi1, CD133, CD44, Sox2, OCT4 and so on [6, 7]. Emerging evidences AG-881 manufacturer showed that these stem-cell-associated markers correlate with tumorigenesis, progression and metastasis, and may be as potential diagnostic markers for various human tumors [8–15]. Bmi1 is an oncogenic member of the polycomb group proteins involved in the self-renewal and differentiation of stem cells. The expression of Bmi1 mRNA has been shown to be a good marker to support the diagnosis of breast cancers in surgically resected specimens [8]. Likewise, CD133, a transmembrane glycoprotein which was first recognized in human hematopoietic stem cells, is considered the most representative marker to isolate CSC from lung cancer [9]. Recently, Moreira et al.