Methods: Hansen’s solubility parameter study was carried out in screening of a suitable carrier and solvent system. Subsequently, SLNs were prepared by solvent diffusion evaporation method and investigated for particle size, polydispersity index (PDI), zeta potential (ZP), entrapment efficiency (EE) and drug release behaviour.
Results: Variations BIRB 796 in vitro in SLN composition resulted in particle sizes between 170 and 810 nm and ZPs between 8 and 14 mV. The maximum EE was found to be 26.3% with particle size of 188.8 nm. SLN can sustain the release of drug for up to 15 h and
it shows Higuchi matrix model as the best-fitted model. SLNs were stable without aggregation of particles under storage conditions.
Conclusions: The results of this study provide the framework for further study involving the SLN formulation for hydrophilic drug molecule.”
“Post-translational Givinostat modifications (PTMs) located on the activation peptide (AP) of recombinant FIX (rFIX, BeneFIX (R)) and plasma-derived FIX (pdFIX, Betafact (R)) have been investigated by mass spectrometry to review the structural differences between these two products. Three major structural differences were pointed out. rFIX contains a low amount of phosphorylated and sulphated AP (4% for rFIX vs. 70% for pdFIX); rFIX N-glycans are only sialylated in the a2-3 linkage, whereas pdFIX N-glycans contain both type
of a2-3 and a2-6 linkages, and rFIX does not contain any sialyl LewisX glycoantigens contrary to pdFIX. These variations might participate in the in vivo potential different behaviours of the two molecules.”
“Object. Patients with moyamoya who are younger than 2 years of age represent a therapeutic challenge because CHIR-99021 of their frequent neurological instability and concomitant anesthetic risks. The authors report their experience with pial synangiosis
revascularization in this population.
Methods. The authors reviewed the clinical and radiographic records of all patients with moyamoya in a consecutive series of patients under 2 years of age, who underwent cerebral revascularization surgery using pial synangiosis at a single institution.
Results. During a 12-year period (1994-2005), 34 procedures (bilateral in 15 patients, unilateral in 4) were performed in 19 patients younger than 2 years (out of a total of 456 procedures in 240 patients). Eighteen of these patients presented with either stroke or transient ischemic attack. The average age of the 19 patients at first surgery was 1.4 years (range 6 months-1.9 years). Unanticipated staged operations occurred in 3 patients, due to persistent electroencephalographic changes during the initial surgery in 2 cases and due to brain swelling during the procedure requiring ventriculostomy in the other. There were 2 perioperative strokes; both patients had postoperative seizures but made clinical recoveries.