In this regard, the discovery of similar interactions between C. Selleck A-1210477 neoformans and Acanthamoebae castellanii and Dictiostelyium discoidum and murine macrophages [12, 13] have led to the hypothesis that the ability of C. neoformans to survive in mammalian cells evolved accidentally, perhaps from interactions with soil predators [11, 14, 15]. A corollary of this hypothesis is that the interactions of C. neoformans with cells from any mammalian species should be similar. In this study, we explore this corollary by studying C. Captisol cell line neoformans

interactions with human peripheral blood monocytes and show that these are similar to those described for murine macrophages. Results C. neoformans replicates and sheds polysaccharide in human peripheral blood monocytes C. neoformans replicated in HPBM cells at similar rates to extracellular C. neoformans, that is, every 2 to 3 h (Figure 1, See additional file 1: Movie 1). To investigate whether polysaccharide-filled vesicles formed following HPBM incubation with C. neoformans, HPBMs with and without ingested C. neoformans cells were permeabilized and incubated with conjugated Alexa 546-18B7, which binds GXM. The cells were then examined

in a confocal microscope for the presence of cytoplasmic vesicles AZD4547 ic50 containing polysaccharide. As in previous studies, vesicles positive for polysaccharide were identified starting at 18 h post infection (Figure 2A). A group of control-uninfected cells gave no positive signal even when overexposed (Figure 2B). Figure 1 Intracellular replication leads to extrusion of C. neoformans phagosome. HPBMs were incubated with C. neoformans strain H99. Following incubation, C. neoformans budding occurred every 2–3 hours as evidenced by the small arrows.

This was followed by extrusion of the C. neoformans phagosomes Liothyronine Sodium as evidenced by the large arrow. Images were collected at 10×. Figure 2 Intracellular polysaccharide shedding by C. neoformans cells. Polysaccharide shedding capacity of C. neoformans strain H99 was tested in HPBMs. Top panel: Intracellular shedding of cryptococcal polysaccharide from C. neoformans cells into HPBMs after 18 h incubation. Bottom panel: HPBMs lacking intracellular cryptococcal cells showed no fluorescence. Bar = 10 μM Cell-to-cell spread and extrusion of C. neoformans by HPBMs To study the occurrence of cell-to-cell spread and extrusion of C. neoformans, we incubated HPBMs with the yeast cells. Following ingestion and subsequent imaging, we witnessed that C. neoformans also spread from host human monocyte to another uninfected one (Figure 3) (See additional file 2: Movie 2), confirming similar observations made in other studies [7–10].