Furthermore, the levels found follow the same pattern of the levels detected for the ground roasted coffee used for brewing, reported in a previous study ( Tfouni et al., 2012), where C. arabica presented higher mean summed PAHs levels than C. canephora. Hischenhuber and Stijve (1987) also did not find correlation between caffeine Stem Cells inhibitor levels and BaP extraction behaviour, with results showing no difference in extraction between canephora and arabica coffees. Results in Fig. 1 also show that filtered coffee, for both cultivars, presented higher mean summed PAHs levels than the boiled brewed coffee.

Although, taking in consideration the caffeine levels and the complex formation, it would be expected otherwise, since boiled coffee presents higher caffeine content than the filtered one (Camargo & Toledo, 1998). Kruijf et al. (1987) analyzed BaP in coffee brew samples prepared by filtration (using a coffee maker) and boiling (addition of boiling water and heat at 90 °C for 15 min). As result there was no difference in the levels detected in both procedures: 0.0008 μg/kg for filtered coffee and 0.0010 μg/kg for boiled. Camargo and

Toledo (2002) evaluated PAHs levels in coffee brew samples prepared from commercial coffees available in Brazil. Authors detected higher PAHs levels in brews prepared by boiling than by filtration. There was no correlation between the PAHs levels detected and the coffees roasting degree. A high variability

of the check details results within the same cultivar and roasting degree, submitted to the same brewing procedure was verified. Although the formation of a caffeine-PAH complex could facilitate PAHs transfer from the ground roasted coffee to the brew, the caffeine levels in the beverages do not seem to influence the transfer. PAHs levels present in the coffee brew Y-27632 in vivo samples analyzed may be considered low when comparing with the maximum permitted levels in the Brazilian regulation or with those established in Europe for different foods (CEC, 2011). It is expected that these levels would not affect the intake of PAHs by the Brazilian population; however, it is important to have and provide information related to potentially carcinogenic compounds in highly consumed food. Financial support from CNPq (477865/2008-9) and scholarship from PIBIC/CNPq-Brasil are gratefully acknowledged. “
“Coalho cheese is a typical Brazilian food that has been produced from raw or pasteurized milk in the Northeastern Region for over 150 years. This product possesses high commercial value due to the simple technology applied during its manufacture, high yield, and good acceptance by the consumers (Silva, Ramos, Moreno, & Moraes, 2010).