Deurenberg RH, Vink C, Oudhuis GJ, Mooij JE, Driessen C, Coppens G, Craeghs J, De Brauwer E, Lemmen S, Wagenvoort H, et al.: Different clonal complexes of methicillin-resistant Staphylococcus aureus are disseminated in the Euregio Meuse-Rhine region. Antimicrob Agents Chemother 2005,49(10):4263–4271.CrossRefPubMed 40. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG: Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 2000,38(3):1008–1015.PubMed 41. Peeters E, Nelis HJ, Coenye T: Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods 2008,72(2):157–165.CrossRefPubMed 42.

Francois P, Koessler T, Huyghe A, Harbarth S, Bento M, Lew D, Etienne Protein Tyrosine Kinase inhibitor J, Pittet D, Schrenzel J: Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay. J Clin Microbiol 2006,44(5):1892–1895.CrossRefPubMed Competing interests The authors declare that they have no competing interests. Authors’ contributions SC carried out the biofilm measurement experiments and performed MLST, collected data and drafted the manuscript. RHD carried out the spa typing/BURP and participated in the design of

the study. MLLB Fludarabine determined the agr types by a real-time multiplex PCR, helped with the statistical analysis and helped to write the manuscript. PB revised GDC-0994 molecular weight the manuscript critically. CN revised the manuscript critically. EES

conceived of the study and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript”
“Background Approximately 600,000 Americans suffer from venous leg ulcers (VLU), which are extremely costly to manage and produce significant suffering [1]. Hippocrates believed that VLU were the bodies way to vent “”evil humors”" and advocated such ulcers should not be treated. His philosophy was that such ulcers should be allowed to express these evil humors naturally [2, 3]. In spite of Hippocrates’ beliefs, the modern clinical goal is to treat and cure VLU. Venous insufficiency is becoming epidemic with almost half of all females Rucaparib solubility dmso and one quarter of all males estimated to suffer from this disease [4]. It is generally agreed that chronic venous disease (CVD) is caused by persistent venous hypertension in the lower extremities stemming from a decay in the efficiency and performance of one-way valves in perforating, superficial or deep veins. Venous hypertension in the extremities, results in clinical changes leading from edema and pain (exacerbated upon standing for long periods of time) through lipodermatosclerosis, hyperpigmentation, hyperkeratosis and ultimately to a proclivity for the development of chronic VLU [1]. As the underlying pathology associated with CVD develops, ulcers typically start when the skin, in the area of fluid accumulation, becomes physically injured (e.g. cuts and abrasions).