15 ELD is also used to develop nano

structure which is used for the crystal growth of the collagen fibres at cathode, so it has vast application in osteotherapy Angiogenesis inhibitor and bio-compositing enamels16 and coating with self assembled amelogenin and calcium phosphate and also used to study bone marrow stromal cell attachment.17 From the above discussion we can conclude that tissue engineering is easier through nanotechnology using nanophase materials in comparison of conventional methods (Fig. 4) and is used in many of the fields for different purposes. Techniques used are as: (i) Electrospinning help to improve adhesion and expansion of hematopoietic stem/progenitor cell at animated nanofiber mesh18 and in Bone marrow these acts as efficient captor and carrier for hematopoietic stem cells.19 (ii) Soft lithography is used in regulating the distribution, alignment, proliferation, and morphology of Human Mesenchymal stem cells,20 initiation of differentiation of embryoid bodies see more of greater uniformity in cell culture in vitro,21 ease to study the growth and differentiation of human Embryonic Stem

Cells under defined conditions and homogeneous aggregation of human embryonic cells.22 (iii) Photolithography to maintain the cells to be in the grooves not ridges and maintaining uniform shape and it also have affects the rate of lipid production and thus differentiation of cells to adipocytes.23 Techniques used are as: (i) Electrospinning helps in cell differentiation, orientation and behaviour like embryoid bodies will differentiate into mature neural lineage cells including neurons, oligodendrocytes, and astrocytes when they will be cultured on polycaprolactone,24 poly (l-lactic acid) nanofibers isothipendyl neural stem cells differentiation is more7 (Yang F et al; 2005). (ii) Replica moulding helps in maintaining cell shape

and behaviour e.g. bovine aortic endothelial cells can be cultured with higher cell alignment frequency and smaller circular index when they are culture on “Poly(glycerol–sebacate) on sucrose-coated microfabricated silicon”25 (iii) Microcontact printing helps to form synaptic connections on defined protocol with polystyrene and polydimethylsiloxane26 also rat hippocampal neurons when cultured with silicon oxide showed resting potential and after 1 day of culture they become capable to reach action potential.27 Techniques are as: (i) Photolithography used to maintain cell behaviour e.g. Chondrocytes isolated from avian sterna were cultured on micropatterned agarose gel which acts as biomomicked scaffolds and helps in maintaining chondrogenic phenotype28 (ii) Replica moulding helps to maintain controlled microenvironment and is integrated with inverted microscope to monitor real-time for cell size change in articular chondrocyte.