001) and CA4-DG (P<0.001). Importantly, we found a positive effect of age on MD in CA2-3 (P<0.001) and fimbria (P<0.001) as well as a negative age effect on FA in the subiculum (P<0.001). Multiple regression analyses revealed that the best overall predictors of age in the hippocampus were MD in the fimbria and volume of CA2-3, which explained 73.8% of the age variance. These results indicate that age has an effect both on volume and diffusion tensor imaging measures in different subfields,

suggesting they provide complementary information on age-related processes in the hippocampus. (c) 2013 Wiley Periodicals, Inc.”
“Prolonged thrombocytopenia is a frequent complication after allogeneic hematopoietic AMN-107 stem cell transplantation (allo-HSCT); however, its pathogenesis has remained obscure. In the present study, we used flow cytometry to determine the SB273005 purchase frequency of bone marrow megakaryocytes (MKs)

and MK ploidy distributions in allo-HSCT recipients with or without prolonged thrombocytopenia (n = 32 and 27, respectively) and healthy volunteers (n = 13). In addition, the expression of c-Mpl in MKs was measured. The results indicate that the proportions of MKs in marrow mononuclear cells or the percentages of CD 110(+) MKs in total MKs did not significantly differ between the 3 groups; however, in a comparison of nonthrombocytopenic allo-HSCT recipients to healthy volunteers, the allo-HSCT patients who had prolonged thrombocytopenia exhibited significant shifts toward low ploidy cells (left shift), which were accompanied by a marked increase in <= 8N cells (P =.036 and P <.001, respectively) DNA Damage inhibitor and significant decreases

in 16N cells (P <.001 and P <.001, respectively) and >= 32N cells (P =.01 and P <.001, respectively). These results indicate that there were more immature MKs in allo-HSCT recipients who had prolonged thrombocytopenia, in comparison to nonthrombocytopenic allo-HSCT recipients and healthy volunteers. We conclude that prolonged thrombocytopenia and slow platelet engraftment after allo-HSCT may be related to a reduction in ploidy and an immaturation of MKs. Biol Blood Marrow Transplant 17: 274-280 (2011) (C) 2011 American Society for Blood and Marrow Transplantation”
“To report a live birth resulting after strontium chloride (SrCl2) oocyte activation in a couple with complete fertilization failure or low fertilization rates following intracytoplasmic sperm injection (ICSI) of frozen-thawed testicular spermatozoa.\n\nThe couple underwent ICSI of frozen-thawed testicular spermatozoa. After ICSI, the oocytes were artificially activated by SrCl2 because the results of fertilization were not satisfactory in the previous cycles. The main outcome measures were fertilization, pregnancy, and birth.\n\nIn the first and second cycles performed previously at another clinic, fertilization rates were 9.