(C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 121: 2559-2566, 2011″
“Polylactic-co-glycolic nanocapsules, loaded with nanosized magnetic particles and Selol (a selenium-based CX5461 anticancer drug), were successfully prepared by the precipitation method. Maghemite (gamma-Fe2O3) nanoparticles were incorporated into the nanocapsules using a highly stable ionic magnetic fluid sample. The obtained nanocapsules presented no agglomeration, negative surface charge while revealing a narrow monomodal size distribution. All the nanocapsule formulations exhibited a good physical stability at 4 degrees C during 3 month storage period. The in vitro antitumoral activity of

Selol-magnetic nanocapsules was this website assessed using a murine melanoma cell line. The influence of nanocapsules on cell viability was investigated by spectrophotometric assay. The results demonstrated that Selol-loaded magnetic nanocapsules (at 100 mu g/ml/5 x 10(9) particle/ml) showed antitumoral activity of 50% on melanoma cells (absence of magnetic field). These results clearly indicate that the loaded nanocapsules represent a novel and promising magnetic drug delivery system suitable for cancer treatment via the active drug and magnetohyperthermia. (C) 2011

American Institute of Physics. [doi: 10.1063/1.3556950]“
“Three-phase partitioning (TPP) was used to partially purify protease from the latex of Calotropis procera (C. procera). To optimize the TPP for protease isolation a ratio of crude extract to t-butanol, percent saturation

of (NH(4))(2)SO(4), and the cycle of TPP was required. The highest proteolytic recovery (first cycle) of 182% with a purification of 0.95 folds was obtained at the interphase of the system comprising the ratio of the crude extract to t-butanol of 1.0:0.5 with the presence of 50% (NH(4))(2)SO(4). The second cycle of TPP was prepared by adding of (NH(4))(2)SO(4) up to 65% (w/v) to the bottom phase obtained from 30% (NH(4))(2)SO(4)-1.0:0.5 system of the first TPP. A purification of 6.92-fold was achieved with about 132% activity recovery. SDS-PAGE and zymography profiles revealed the substantial isolation of protease from C. procera EPZ5676 latex by the TPP. The molecular weight of major protease was found to be around 28 kDa. The present study shows high interesting outcomes and could be used as a primary purification process in comparison with existing literature’s values. (C) 2010 Published by Elsevier B.V.”
“A novel chiral azobenzene polyurethane (CAPU) was prepared from chromophore, chiral reagent L(-)-tartaric acid and toluene diisocyanate (TDI). The chemical structure and the thermal property were characterized by UV-Vis spectrum, FT-IR, (1)H NMR, circular dichroism (CD) spectrum, differential scanning calorimeter (DSC) and thermogravimetric analysis (TGA).