sali narum. Even so, Nab. magadii lacked genes related to these encoding the minor gas vesicle protein as well as the regulators. The fuel vesicle gene clusters of Nab. magadii and Htg. turkmenica have been highly much like one another and appeared to consist of a distant homolog from the Hbt. salinarum gvpI gene. Nevertheless, these genes encode putative proteins with an N terminal extension of much more than 200 residues not discovered in GvpI of Hbt. salinarum. Additionally, the fuel vesicle clusters of Nab. magadii and Htg. turkmenica contained an include itional gene that was absent in Hbt. salinarum. Nab. magadii also contained a gene encoding a hemAT sort aerotactic transducer with a putative globin coupled sensor pro tein comprising of the globin fold domain and a methyl accepting chemotaxis transducer domain.
These traits, additionally for the os motic adaptation mechanisms mentioned above, could perform a purpose inside the survival of Nab. magadii in its nat ural surroundings. Other capabilities likely to facilitate the adaptation of Nab. magadii to its niche incorporated genes encoding puta tive mechanosensitive ion channels selleck chemical that afford protection towards hypoosmotic shock, chaperone proteins DnaJ and DnaK, a thermosome, and heat shock proteins that may take part in protein quality manage and cellular response to tension. Nab. magadii also contained 47 genes encoding putative proteins of a variety of sizes having a universal strain protein domain. Certainly one of these genes appeared to type an operon that has a gene encoding a putative GCN5 relevant N acetyltransferase, and also a similar gene pair was identified in Htg. turkmenica and Nmn.
pharaonis. It truly is attainable the GNAT is involved in the acetylation with the linked universal strain protein in these species. Furthermore, the huge chromosome of Nab. magadii contained genes encoding a superoxide dismutase, two catalases, two alkyl hydroperoxidase like proteins, a carbonic anhydrase, and selelck kinase inhibitor methionine sulfoxide reductases. It is actually possible that these enzymes possess a part inside the adap tation of this haloarchaeon to different oxidative stresses related with power metabolism. Moreover, Nab. magadii contained genes encod ing metal transport proteins as well as a putative copper re sistance protein. Nab. magadii copper resistance protein seems to include fused CopC CopD domains along with a distant homolog of this protein happens in Nmn. pharaonis, but not in other archaea.
These genes might be involved in metal homeostasis within the hypersaline setting that Nab. magadii inhabits. Nab. magadii also encoded DNA methylases, DNA damage fix excinuclease ABC subunits, DNA mismatch fix proteins, and DNA repairrecombination proteins RadA and RadB. Homologs of these genes are discovered in many other archaea and they are pre dicted to be involved in pressure response and keep ing genetic integrity.